![]() ![]() The most abundant species, SCO2217, SCO0930, and SCO2207, corresponded to secreted proteins or lipoproteins of unknown functions whose genes are in the proposed σ U regulon. Peptide mass fingerprinting was used to identify 79 proteins from the rsuA mutant culture supernatant. This mutant secreted a much greater quantity and diversity of proteins than the wild-type strain. Since many of these genes encode probable secreted proteins, we characterized the extracellular proteome of a mutant with high σ U activity caused by disruption of rsuA, the presumed cognate anti-sigma factor of σ U. We have defined a σ U-dependent promoter sequence and used this to identify 22 likely σ U regulon members in the S. Using a green fluorescent protein transcriptional reporter, we have demonstrated that sigU transcription is autoregulated. Uncontrolled activity of the extracytoplasmic function sigma factor σ U blocks the process of aerial mycelium formation in this organism. ![]() The filamentous bacterium Streptomyces coelicolor forms an aerial mycelium as a prerequisite to sporulation, which occurs in the aerial hyphae. ![]()
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